DNA

Part:BBa_M11041:Design

Designed by: Ashton Young   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-15)

pyruvate decarboxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 445
    Illegal NgoMIV site found at 1270
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1175
    Illegal AgeI site found at 1553
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence for the pyruvate decarboxylase of Zymomonas mobilis subsp. mobilis ZM4 was found using the NCBI registry (YP_163095.1):

MSYTVGTYLAERLVQIGLKHHFAVAGDYNLVLLDNLLLNKNMEQVYCCNELNCGFSAEGYARAKGAAAAV VTYSVGALSAFDAIGGAYAENLPVILISGAPNNNDHAAGHVLHHALGKTDYHYQLEMAKNITAAAEAIYT PEEAPAKIDHVIKTALREKKPVYLEIACNIASMPCAAPGPASALFNDEASDEASLNAAVEETLKFIANRD KVAVLVGSKLRAAGAEEAAVKFADALGGAVATMAAAKSFFPEENPHYIGTSWGEVSYPGVEKTMKEADAV IALAPVFNDYSTTGWTDIPDPKKLVLAEPRSVVVNGIRFPSVHLKDYLTRLAQKVSKKTGALDFFKSLNA GELKKAAPADPSAPLVNAEIARQVEALLTPNTTVIAETGDSWFNAQRMKLPNGARVEYEMQWGHIGWSVP AAFGYAVGAPERRNILMVGDGSFQLTAQEVAQMVRLKLPVIIFLINNYGYTIEVMIHDGPYNNIKNWDYA GLMEVFNGNGGYDSGAGKGLKAKTGGELAEAIKVALANTDGPTLIECFIGREDCTEELVKWGKRVAAANS RKPVNKLL

This polypeptide sequence was back translated for E. coli to form this nucleic acid using the free downloadable software called DNA 2.0:


A ribosome binding site (pRBS-SD+7Nde) was also added using DNA 2.0

And two stop codons were added using DNA 2.0

Source

Zymomonas mobilis mobilis ZM4

References

Neale, A. D. (1987). Pyruvate Decarboxylase of Zymomonas mobilis: Isolation, Properties, and Genetic Expression in Escherichia coli. Journal of Bacteriology , 1024-1028.